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Expression levels were calculated using the relative quantification method.
The expression levels of the target genes of the different treatments were normalized to the rpoA housekeeping gene and compared using the relative quantification method.
The expression level was calculated using the relative quantification method.
Expression level was calculated using the relative quantification method (Carleton 2011).
Results were expressed using the relative quantification calculation method in arbitrary units as described in [40].
The results were normalized to β-actin expression and evaluated using the relative quantification method.
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For gene dosage measurements, we used the relative quantification-monocolor analysis from the LightCycler Software 4.0 package (Roche).
The Pfaffl method [ 54] was used for the relative quantification of the transcript accumulation of the genes of interest using L2 as reference gene.
The Pfaff method [ 52] was used for the relative quantification of the transcript accumulation.
The ΔΔCt method [ 39] was used for the relative quantification of mRNA.
The comparative cycle threshold Ct method was used for the relative quantification of gene expression.
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using the relative gear
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using the relative expansion
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