Effect modification by comorbid health conditions was tested using the relative effect modification (REM) index.
To hide data robust to modification of audio amplitude, the watermark is embedded in the DWT domain using the relative relationships among different groups of the DWT coefficients.
Because we used equal amounts of starting material for each qPCR experiment, we were able to use the Relative Quantity (ΔCT) method of analysis, in which no modification of the data is needed to obtain normalized data.
The relative quantification was made using the Pfaffl modification of the ΔΔCt equation, taking into account the efficiencies of individual genes.
Relative quantities were determined using the equation; relative quantity = 2−ΔΔCt.
Modification information is captured in PRIDE using the PSI-MOD (Modifications) ontology (13).
The modification of CNT using the plasma is presented in "Plasma Modification of CNTs".
The first strategy can be applied with minimal a-priori information on the masses (i.e. using a parameter that quantifies an eventual relative modification of the total mass of the structure before and after damage).
The relative expression level of each gene was calculated using the 2- ΔΔCt) method with some modifications [ 59]: ΔΔCT = (CT, Target − CT, Actin) − (CCK, Target − CCK, Actin).
Relative expression was determined using the free access software qBase [ 37] based on a modification of the classic delta-delta Ct method that allows for PCR efficiency correction.
