To compare the amount of STRs in different subsets of genes we therefore used the relative amount of STRs in a gene, i.e. the length of STRs in the gene relative to the length of the gene.
We thus used the relative amounts of the four possible PCR products as a reporter of DNA quality, and therefore suitability in aCGH.
Therefore, the rat data were normalised using the relative starting amounts of cDNA, which was determined using a novel technique recently developed in our laboratory (J. M. Hughes, I. Klaassen, W. Kamphuis, C. J. F. Van Noorden and R. O. Schlingemann, unpublished results).
Transcript quantities were compared using the relative Ct method, where the amount of target normalized to the amount of endogenous control (GAPDH or RNU6) and relative to the control sample is given by 2(–ΔΔCt).
The qPCR data may be used to estimate the relative amount of transcripts using the 2-ΔΔ C T method [ 42] and the β-actin as reference gene.
Using this method, the relative amount of S-nitrosated proteins was shown to increase with CysNO concentration.
The TCI has been used to express the relative amount of crystalline material in cellulose, and it can be defined using the FT-IR using the absorbance at A1430/A898 [10].
The method used to calculate the relative amount of cDNA was described by Liu and Saint [30], and the relative efficiencies for individual amplification were calculated using the LinRegPCR program [31].
Quantitative analysis of EXAFS spectra was used to estimate the relative amount of ligation by sulfur (as sulfide) or oxygen atoms, or as a mixture of both atoms.
First, a phylogram from Hackett et al. (2008) was used to estimate the relative amount of genetic change of each order from the origin of birds and suggest placement of orders on the map in the present day.
