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Quantification cycle (Cq) values were determined using the "regression" mode.
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These regressions and simple linear regressions were performed using the regression wizard in SigmaPlot 13.0 (Systat).
In case of RF the classification mode has a slight edge while for NNs and SVMs the regression mode wins.
The gel image analysis workflow included spot detection, spot normalization using local regression mode available in the software and finally spot matching and differential expression analysis.
The main statistical analysis was made using binary logistic regression (mode enter) carried out on a regular basis with subsequent bootstrapping (1000 bootstrap replications, 95% percentile method and simple sampling) 31 to provide more accurate SE and CIs.
Comparative cycle threshold (Ct) values for each target gene were calculated by Bio-Rad CFX Manager 2.1 software (Bio-Rad Laboratories Inc). using regression mode and relative copy number ratio was measured by the E ΔCt method over the reference gene LAMA1, where E is the PCR efficiency calculated by standard curves generated from dilution series of calibrator gDNA, as previously described [ 15].
The significant predictor variables for self-reported function (WOMAC) and reported weekly duration of physical activity using multiple regression modes are shown in Table 4.
In Table 2 the dependent variable is labor market match defined using the mode method.4 Regressions are estimated separately for men and women and relative risk ratios are presented.
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