Molecular phylogenetic trees were inferred using the regions that were unambiguously aligned with no gaps.
Touchdown PCR (95°C, 10 min; 10 cycles of 95°C for 30 s, 65°C for 30 s and 72°C for 45 s, with a decrease in the annealing temperature of one-half degree per cycle; 35 cycles of 95°C for 30 s, 55°C or 50°C for 30 s and 72°C for 45 s, 3.75 or 2.5 m m final Mg2+ concentrations) was performed on bisulfite-treated DNA with primers designed using the regions that flank the CpG-containing sequences.
To this end, multiple genome databases (HTGS, GSS, WGS; 36 species in total) were queried and sequence alignments were carried out using the region that spans the exon 9-intron 9 junction (Figure 1B and Figure S1A).
Motion discontinuities and not the flow estimates itself are used to find the regions that belong together.
The exact positions of ribosome profiling reads on the RNA can be used to delineate the regions that are being actively translated or to discover new functional ORFs (Chew et al., 2013; Guttman et al., 2013; Ingolia, 2014).
The information of variance estimate is used to distinguish between the regions that contain image detail and flat, homogenous regions, that is, regions with high and low variance, respectively.
C. 650 BCE - c. 601 BCE Alcman, Doric Alcmaeon (flourished 7th century bc, Sparta [Greece]) Greek poet who wrote choral lyrics in a type of Doric related to the Laconian vernacular, used in the region that included Sparta.
In fact, these group B meningococci belonging to the A4 lineage were also isolated in some of the regions that used the vaccine on a small scale (13).
The amino acid alignments of the remaining 28 ortholog groups were used to identify the regions that correspond to the P. falciparum PEXEL in the sequences from the other species.
To determine the effects of DISC1 Ser704Cys on functional connectivity to the thalamus, we performed voxel-vise functional connectivity analyses using the thalamic region that showed significantly different long-range FCD as the ROI.
Southern blot analysis using part of the region that is deleted in mutant pro23 as a probe showed that indeed this region is present in the wild-type and mutant pro44, but absent in mutant pro23 (data not shown).
