Sentence examples for using the reference sequence from inspiring English sources
Cuffmerge was then used to merge assemblies into a master transcriptome, using the reference sequence and reference genome specified above.
Sequences were analysed using the reference sequence, GenBank accession number AC090645.
In theory there are two different ways of using the reference sequence to guide the assembly process.
We designed 60-mer oligonucleotides using the Reference Sequence database (RefSeq) [ 26] and the UniGene database [ 27] because of their complementary features.
The BamHI map spans 2,575.30 Mb and comprises 78 optical contigs assembled by a combination of iterative (using the reference sequence: UMD3.1) and de novo assembly techniques.
Aligned reads are clipped within each active region and assembled into a De Bruijn graph, edges weighted by observation counts using the reference sequence as a backbone.
After hand correction of crude sequences in MEGA3, alignments were performed using the reference sequences from SIVmac239 as root (Ac# M33262).
Separate alignments for the exon and intron sequences were also generated using the reference sequences as a guide.
All clean sequencing tags were annotated using the reference sequences which covered all possible CATG+17-nt tag sequences of the genome and transcriptome of B. rapa and those of B. oleracea, respectively using blastn, allowing only a 1-bp mismatch.
Sequence electropherograms from each cloning experiment were aligned and edited in Sequencher v.4.5 (Gene Codes) using the reference sequences to orient the alignment and to trim the ends to the start and stop codons.
The tropism characteristics of each U3 sequence was determined by comparison analysis using the reference sequences (direct repeats, unique region, and 190 bp insertion) first reported by Tomonaga et al. [ 30].
