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The completion of a draft of the genome sequence of melon [ 28] gives us the opportunity to mine SNVs on a genomic scale by using the reference genome for the alignment of short reads obtained by resequencing the variability across the species.
Ideally, we agree that it would have been better to scaffold the A. veronii genome assembly generated de novo from our meta3C library and convert it into a genomic contact map directly, without using the reference genome.
Short reads were aligned using the reference genome hg38 (http://genomeast.igbmc.fr).igbmc.fr
In brief, base substitution patterns of the somatic mutations detected in SCCs were analyzed and visualized with MutationalPatterns using the reference genome set to "BSgenome.Hsapiens.UCSC.hg19".Hsapiens.UCSC.hg19
Two forms of phylogenic trees on genome structures were produced: one (form A) using the reference genome from strains J99, HPAG1, SHI470 or P12 and another (form B) using the reference genome from strains 26695, B38, 51, 52 or G27 (Figure 1a, b).
Using the reference genome as a guide, the length of each 454-FLX read in the SAG A library was extended to ∼500 bp and ∼700 bp from its 5' end.
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Here we used the reference genome for S. cerevisiae S288C, assembly R64-1-1 R64-1-1 R64-1-1Genome DatabaSaccharomyces
Thus, we use the reference genome to align the variants such the same variants are compared.
Thus, we used the reference genome to map piRNA reads for all strains.
Repeats size is for Nymphaea, which was used the reference genome in Blast comparisons.
We used the reference genome build 37.1 as the most comprehensible, widely used and containing the largest amount of large TR to map newly found TR.
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