Sentence examples for using the recommended procedures from inspiring English sources
The fragmented biotinylated cDNA (15 µg) was hybridized onto the MG-U74Av2 GeneChip (Affymetrix), using the recommended procedures for prehybridization, hybridization, washing and staining with streptavidin phycoerythrin (SAPE).
Microarray analysis employed Affymetrix rat genome 230 2 arrays and was performed using the recommended procedures of the manufacturer.
The items on both questionnaires will be scaled and scored using the recommended EORTC procedures [ 38].
The items on both measures were scaled and scored using the recommended EORTC procedures (Fayers et al, 2001).
Amplicon libraries were generated using the recommended workflow procedures from the Ion Fragment Library Kit and Ion Xpress™ Template kit (Life Technologies).
Total RNA (10 μg) was processed by a one-step labeling protocol (Affymetrix), and fragmented cRNA (15 μg) hybridized to the Arabidopsis ATH1 Genome using the recommended standard procedures (45°C for 16 h).
The European Specification EN 15512 recommends testing, however it is not clear whether the shear stiffness obtained using the recommended test procedure is correct.
DNA was purified using Performa DTR Gel Filtration Cartridges EDGE BioSystemss), following the recommended procedure.
Second, the inelastic analyses were carried out in accordance with the recommended procedure by using the measured results of oscillating temperature distribution.
Transfections were performed using Lipofectamine 2000 (Invitrogen) according to the recommended procedure.
The Brazilian version of K-SADS-PL was developed from the original English version7 using recommended procedures for translation, back-translation and cultural adaptation [ 13- 16].
