Sentence examples for using the recombinant enzymes from inspiring English sources

We further analyze ChoK and EtnK kinetic activities for ChoKα1 and ChoKβ isoforms using the recombinant enzymes.

While it is acknowledged that the measurement of gene expression in the brain is relatively difficult and can only be done in autopsy tissue, it would be helpful to incorporate the study of CYP3A43 mediated metabolism using the recombinant enzyme.

Therefore, a detailed functional characterization of α- l-arabinofuranosidase II using the recombinant enzyme with its mutants would be interesting because all the enzymes belonging to GH43 have the same holding and catalysis in the same inverting mechanism even though the enzyme activities are different.

We found a reaction using the recombinant spruce enzyme as well, which is not yet quantified due to our limited resources.

To exploit this hypothesis, we used the recombinant human enzyme, Cz, which hydrolyzes the glucose moiety of mammalian GlcCer.

The biochemical properties were investigated by using the purified recombinant enzyme.

The recombinant enzymes used in this study were expressed according to the protocol involving optimized conditions as described previously (Li et al. 2016).

A functional analysis of the recombinant enzymes when using GDP (C10), FDP (C15), and GGDP (C20) as substrates indicated that only GGDP was converted into several reaction products in the presence of MgCl2.

After purification, the activity of the recombinant enzymes was tested using the methylene blue method.

The ability of β-glucosidase to hydrolyze the lactose in milk at 65°C for 30 min was tested using different amounts of the recombinant enzyme and then analyzed by HPLC which showed that the lactose in milk can be hydrolyzed into glucose and galactose.

The cell homogenate was centrifuged at 4°C for 15 minutes at about 15000 g using a Sorvall centrifuge and the clear supernatant used as source of the recombinant enzyme.