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The multiple-round in vitro transcription assay was in principle performed using the recently described system (Holátko et al. 2012).
It was the base for determination of their surface chemistry (including stoichiometry and contaminations) based on the atomic sensitivity factor (ASF) approach [9] using the recently described procedure [5, 6].
Here, we have further investigated the three-dimensional structure of the 4q subtelomeric region using the recently described 3C technique.
This led us to evaluate the outcome of MEK inhibitors-enhanced HCV replication on virus production using the recently described HCV JFH-1 cell culture system [16].
Similar findings were observed using the recently described protocols to differentiate HSCs based on their expression of the SLAM family receptors CD48 and CD150[30] (data not shown).
Firstly, Siebenga et al choose to model the aa changes they have observed onto the crystal structure of Norwalk virus [4], whereas our homology modelling study was performed using the recently described crystal structure for the virus VA387 [24].
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As donors we used the recently described yellow fluorescent protein variant SYFP2 [10] or the orange fluorescent proteins mKO [26] and mOrange [16].
In this report, we used the recently described OPEN zinc finger engineering method to rapidly generate ZFNs that can modify endogenous zebrafish gene targets with high efficiency.
For this matter, we used the recently described fluorescence self-quenched substrate DiE-GSSG [12] that is easier to synthesize and exhibits a much higher signal than the previously described substrate di-aminobenzoyl-GSSG [13].
For staging of Aβ-pathology, we used the recently described four phases of β-amyloidosis in the MTL [ 34].
We used the recently described pseudo-gene ratioing technique to estimate the amount of mtDNA remaining in the candidate A549 ρ0 cell cultures (Methods) [ 21].
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