Thus, by using the RecA-GFP fusion protein we have been able to segregate the RecA function on swarming promotion from the other RecA functions.
Our results using the recA-defective strain and the application of a recA-downregulating agent (novobiocin) suggest that the effectiveness of photoinactivation depends in part on RecA activity, thus supporting the hypothesis that RecA inhibition is a potential therapeutic adjuvant in combination with photodynamic approaches.
The RecA sequence was aligned to 60 homologous sequences from the Proteobacteria and 317 positions were used to build the tree.
The recA DNA sequence of the bacterial isolate (GenBank accession no. AJ786367), subjected to BLAST analysis (http://www.ncbi.nlm.nih.gov/BLAST), showed >99% homology with the recA sequence of the B. cenocepacia reference strain LMG 18829 (GenBank accession no. AF143784).
Using the combined approach of recA sequence analysis and mAb typing, it was possible to accurately classify all P. acnes isolates into phylogroups IA (IA1 and IA2), IB, IC, II, or III.
A phylogram for analyzing the genetic similarity between clinical and environmental isolates of the genus Stenotrophomonas (all strains of Table 1) was built using the concatenated fragment sequences of the genes atpD, gapA, guaA, ppsA, nuoD, and recA.
A search for a residual recA in the Rma and Vok genomes using this sequence and the Escherichia coli recA sequence revealed that the Rma genome contains a DNA fragment similar to recA between Rmag_0799 (ABC transporter related, Figure 2A) and Rmag_0800 [diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6- 5-phosphoribosylamino) uracil redeaminase/5-amino-6- 5-phosphoribosylaminoes not.
The RecA-like SAR1 subfamily appears be a sister group to the traditional bacterial RecA proteins (Figure 1) and thus we use the prefix "RecA-like" for it.
Data were analyzed using the ΔΔCt method with recA as the reference [ 57].
The partial recA genes were sequenced using the M13 universal and reverse primers.
