Protein concentration was estimated by densitometric analysis of SDS gel bands using the Quantity One analysis software (Bio Rad, Hercules, CA, USA) and by the use of the extinction coefficient at 280 nm (77,015 M−1 cm−1) determined by urea denaturation compared with the theoretical extinction coefficient based on the amino acid sequence.
The relative density was quantified using the Quantity One analysis system (Bio-Rad, California, USA).
Subsequently, band intensities were quantified by densitometry, using the Quantity One 4.6.2 analysis software (BioRad).
Larval volumes were quantified from 16-bit grayscale pictures using the Quantity One volume analysis tool (Bio-Rad).
The data was analyzed using the Quantity One 1D analysis program, version 4.1 (Bio-Rad, Hercules, CA).
Protein band intensities from western blot analyses were quantitated using the Quantity One 1D analysis software v.4.6.0 (Bio-Rad).
For quantification purposes, densitometric measurements were performed using the Quantity One image analysis software for Windows (Bio-Rad, Alfred Nobel Drive Hercules, CA, USA).
The sequences were aligned and analysed using Vector NTI Suite 9 (Invitrogen, Carlsbad, Calif .. Using the Quantity One 1D Analysis software package (BioRad, Hercules, Calif ., the agarose gel images were analysed and allelic sizes estimated.
Protein bands were characterised using the Quantity One 1-D analysis software (Bio-Rad Laboratories).
Blots were visualized by ECL technique and quantified by using the Quantity One 1-D analysis software (Biorad).
