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Serum samples were analyzed to evaluate markers of complement activation (C3a), proinflammatory cytokines (tumor necrosis factor-α, interleukin-1β, and interleukin-6), and coagulation (thrombin-antithrombin complex [TAT]) using the quantitative sandwich enzyme immunoassay technique.
The level of VEGF165 in preoperative sera of gastric cancer patients and healthy donors was assayed using the quantitative sandwich enzyme immunoassay technique.
Serum levels of eight endothelial factors that have been shown to be implicated in SSc (VEGF, placenta growth factor (PlGF), sVCAM, angiopoietin-2, endoglin, endostatin, endothelin-1 and Tie-2) were measured by using the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) technique (Quantikine kits; R&D systems) [ 11- 17, 18, 18, 26- 28].
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It used the quantitative sandwich enzyme immunoassay technique where only free, unbound forms of the growth factor are detected.
This immunoassay used the quantitative sandwich enzyme technique, which is based on a monoclonal antibody specific to the human FGF19.
The assays employ the quantitative sandwich enzyme immunoassay technique using recombinant human IL-6 and TNF- α, with antibodies raised against the recombinant proteins, respectively.
In brief, these assays employ the quantitative sandwich immunoassay technique.
Both assays employ the quantitative sandwich enzyme immunoassay technique.
The ELISA assays employ the quantitative sandwich enzyme immunoassay technique (22, 23).
MAb 1F11 and 2F10, which we subsequently used to develop the quantitative sandwich ELISA, both recognized human native milk OPN.
Serum levels of TNF-α were quantified using an ELISA that follows the quantitative sandwich immunoassay technique.
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