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Quantitative real time PCR was performed using the quantitative SYBR Green PCR kit (TaKaRa Bio, China).
The NLP pellets were washed twice with HEPES buffer using the same centrifugation steps.
Much higher density of the Au NPs were found on ITO substrates using the centrifugation method.
Background readings were corrected using the centrifugation supernatants from blank nanoparticles.
Virus-bound plates were prepared using the centrifugation method.
The free melatonin concentration was determined using the ultrafiltration-centrifugation technique (Microcon 10,000 KDa, Millipore) [24].
Peripheral blood mononuclear cells were isolated using the Ficoll Hypaque density centrifugation technique.
Following centrifugation, protein concentration was determined using the Bradford assay.
After centrifugation at 12000×g centrifugation for 5 min, the protein content of the supernatant was determined using the Quick Start™ Bradford assay (Bio-Rad, Hercules, CA).
After centrifugation, the ATP concentration was determined on the supernatant using the ATP Determination Kit (Invitrogen).
No significant difference in hMSC growth and characteristics was observed between cells cultured using either the manual centrifugation process step or the automated non-centrifugation process step, in which residual dissociation agent is carried over.
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