Exact(6)
Image analysis was carried out using the Quantitation module of Volocity (PerkinElmer).
SILAC [24] studies can be processed by initial database searching with Mascot and then using the quantitation toolbox in Mascot Distiller (Matrix Science Inc).
Threshold cycle (Ct) and melting curves were acquired by using the quantitation and melting curve program of the Bio-Rad iCycler iQ system (Bio-Rad Laboratories).
For the LC3B-, GABARAP- and SQSTM1-positive dots count, we performed intensitometric analysis of fluorescence using the Quantitation Module of Volocity software (PerkinElmer Life Science, I40250).
Measurements of seedling and root length, number of cells per mm in root cell rows, cross-sectional area of nucleoli, and proportion of nucleolar granular component (GC) were obtained from the digital images using the quantitation software "QWin Standard" (Leica Microsystems, Germany).
As shown in Figure 3D, stimulation of MAPK15 expression in inducible HeLa cells led to a ~6.8-fold increase in the number of autophagic structures, as compared with uninduced cells (quantification performed through unbiased analysis using the Quantitation Module of Volocity software).
Similar(54)
Quantitation was performed using the Q3 quantitation tool [29].
The quantitative-PCR data was quantified using the comparative quantitation analysis program of MxPro Q-PCRanalysis software version 3.0 (Stratagene).
Quantitation of target RNA, normalized with an endogenous control (Cyclophilin), was performed using the absolute quantitation method (Applied Biosystems).
DapG2 gene primers and probe (Additional file 1: Table S2) were used to quantitate genomic DNA using reference B. anthracis DNA using the absolute quantitation method (Life Technologies, Carlsbad, CA).
Images were then analyzed using the Volocity Quantitation module (Perkin-Elmer, Waltham, MA).
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