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Data were cleaned using the quality control (QC) pipeline developed by the eMERGE Genomics Working Group.
As this effect is difficult to simulate without knowing the exact bias, we decided to obtain an empirical error rate of pooled samples after using the quality control measures mentioned above: trimmed reads, sequencing quality, and minor allele count.
Quality assessment of the normalized data file was conducted using the quality control function in CLC Bio.
Quality assessment of the normalized datafile was conducted using the quality control function of transcriptome analysis in CLC Bio.
Sequence reads were obtained and their quality was analyzed using the quality control metrics provided by the FastQC pipeline (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).ac.uk/projects/fastqc/
Genotype data were cleaned using the quality control (QC) pipeline developed by the eMERGE Genomics Working Group (Turner et al. 2011; Zuvich et al. 2011).
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The quality and reproducibility of the assay was determined using the quality controls provided with the kit.
The reproducibility of the assay for individual cytokine was determined using the quality controls provided with the kit.
We thank the Australian and New Zealand Intensive Care Society (ANZICS) Centre for Outcomes and Resource Evaluation (CORE) for the possibility to use the quality control data for scientific purposes.
Although a correct cross-calibration is guaranteed using the quality-control procedure described above, differences in SUV quantification may still occur between centres as a result of differences in the reconstruction and data analysis methodology used.
In this study, the support vector machines, which have an increasing rate of use in pattern recognition area, are used in the quality control of DNA sequencing data.
More suggestions(15)
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