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The serum S100A12 levels determined in our population, using the prototypic ELISA described above, were lower compared to IBD-oriented studies, using different ELISA assays [ 7, 8, 10].
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To address these issues, we have developed a consistent numbering scheme (see Methods) using the prototypic PKA as the frame of reference.
We therefore used the prototypic histone deacetylase inhibitor TSA to modulate MT expression.
We have here used the prototypic polymeric drug carrier pHPMA to validate the potential of this targeted combination regimen.
The calibration curve of ROS using the proposed ELISA is shown in Figure 3B.
Cytokine level was determined using the Cytoscreen® ELISA Kit (Biosource).
TNFR1 was measured using the TNFR1 ELISA.
ELISA was performed using the BrdU ELISA kit (Roche Diagnostic Corp., Tokyo, Japan).
Samples positive by ELISA were confirmed by using the inhibition ELISA method (8 ).
Concentration of HIF-1 α protein in cell culture lysates was quantified using the quantitative sandwich ELISA immunoassay (Invitrogen, HIF-1 α ELISA Kit; EHIF1A).
ELISAs were carried out by using the DS2 Automated ELISA workstation (DYNEX Magellan Biosciences).
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