Sentence examples for using the protocols suggested from inspiring English sources

Magnetic beads conjugated with the cell-type specific markers CD11b, CD4 (L3T4), B220, or CD8 (Ly-2) (Miltenyi, Auburn, CA) were used to purify individual cell types using the protocols suggested by the manufacturer.

cRNA samples were prepared and hybridized to the array (Affymetrix® Hg-U133A™); its signals were then scanned using the protocols suggested by the manufacturer.

If treated using the protocol suggested by Moses et al. [30], the remaining S0 impurity (~40% of the original impurity) would still constitute ~3.9% of the complete oxidation products.

Transfections were performed using Lipofectamine™ 2000 (Invitrogen) using the protocol suggested by the manufacturer.

Plasmids were mixed at 0.5 μg per well and transfection was done using the Lipofectamine Plus transfection reagents (Invitrogen) using the protocol suggested by the supplier.

The high fidelity polymerases Phusion (New England Biolabs) or iProof (Bio-Rad) were used according to the protocols suggested by the manufacturer to generate all cloning insert fragments.

The immunoprecipitation and immunoblot methods used were similar to the protocols suggested by the manufacturer (Santa Cruz Biotech, CA, USA).

The results obtained using the inactivation protocol suggested that the R-type voltage-gated Ca2+ channel sub-type was expressed in the somatic membrane of L5 pyramidal neurons (Fig. 4b).

Genomic DNA was isolated from mouse tail using a kit, and the protocol suggested by the manufacturer (DNeasy Blood & Tissue Kit, Qiagen, USA).

The PCR product was gel purified using the Novagen kit, following the protocol suggested by the manufacturer and digested using NcoI and NotI restriction enzymes.

An alternate laboratory host Corcyra cephalonica Stainton (Lepidoptera: Pyralidae) was used for rearing the culture following the protocol suggested by Lalitha and Ballal (2015).