Genome-wide DNA methylation was assessed using the Illumina (San Diego, CA) HumanMethylation450 Beadchip by the University of Minnesota Genome Center (Minneapolis, MN) using the protocol specified by the manufacturer as previously described [ 26].
Measurement of genome-wide peripheral mononuclear cell DNA methylation status of FACHS participants was conducted using the Illumina (San Diego, CA) HumanMethylation450 Beadchip by the University of Minnesota Genome Center (Minneapolis, MN) using the protocol specified by the manufacturer [ 6].
Using the MAC protocols specified in the WiMedia ECMA-368 standard as an example, extensive simulations have been conducted to validate the analysis.
SCE followed the protocols specified by ASTM E1226 and E1515.
Tumor tissue sections prepared from formalin-fixed, paraffin-embedded tumor tissues were used for IHC studies according to the protocol specified in the SignalStain™ Cleaved Caspase-3 (Asp 175) IHC kit (Cell Signaling, Beverly, MA).
To prevent weight regain the protocol specified use of partial meal replacement as a first option (exchanging one or two daily meals with a 0.58 MJ meal replacement, Cambridge Weight Plan, Northants, UK) if the patient's weight had increased by more than 2 kg since the last visit (86% reported using partial meal replacement at least once).
The Frame Slotted Aloha protocol is widely used due to its simplicity and is the protocol specified for the Class 1 Gen 2 EPC standard [11].
Genotyping was carried out using the Illumina GoldenGate technology (San Diego, CA, USA), according to the protocol specified by the manufacturer.
Originally the protocol specified that compliance would be determined using data from an electronic device built into the helmet to measure compliance with wear.
The protocol specified that simple language should be used throughout and that the benefits of research should be highlighted through the use of examples.
