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The oligomer identification has been performed using the protocols reported in [40] and [43].
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Transient expression assays in Nicotiana benthamiana were performed using the protocol reported previously (Li, X. 2011).
In addition, all patients in both groups were managed by tight glucose control, using the protocol reported by Van den Berghe [32],[32].
The AROS model was subjected to molecular dynamics simulations by using the protocol reported in the Methods.
The model obtained for AROS was subjected for 15 ns to molecular dynamics simulations using the protocol reported above to assess its conformational stability.
In situ hybridization was performed using the protocol reported by Martinez and co-workers [ 82] with several modifications.
Radiation on rabbits was performed by radiotherapists in Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong, using the protocol reported in our previous study [ 8].
In addition, all patients in both groups were managed by tight glucose control, using the protocol reported by Van den Berghe [ 32],[ 33].
HDAC8 overexpression was achieved by transient transfection of 0.5 μg HDAC8 construct or empty vector (OriGene, Rockville, MD, USA), using the protocol reported before for HDAC3 and HDAC6.
A total of 635 EST derived markers developed within the Grain Legumes Integrated Project (GLIP-Food-CT-2004-506223), were tested for polymorphism among the parental lines using the amplification protocols reported by [ 13, 26].
PCR amplifications were accomplished using the protocol previously reported in [34].
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