Sentence examples for using the protocol supplied from inspiring English sources

Total RNA was extracted using the protocol supplied with the Trizol reagent.

A series of high-stringency washes were performed, using the protocol supplied.

TNF-α was quantified by enzyme-linked immunosorbent assay (ELISA), using the protocol supplied by the manufacturer (B&D, USA).

The apoptosis assay was conducted using the protocol supplied by the manufacturer (BD Biosciences, Bedford, MA, USA).

siRNAS were transfected with HiPerfect Transfection Reagent (QIAGEN), using the protocol supplied by the manufacturer for J774 cells transfection.

cRNA for microarray hybridization was prepared from 5 ug of starting RNA using the protocol supplied by Affymetrix (Santa Clara, CA).

The Seleno-Met site was determined and refined using the protocols supplied with the program CNS using a dataset measured at three wavelengths (inflection, peak and remote).

The protein concentrations of pro-MMP-2 were measured using commercially available EIA kit (Daiichi Fine Chemical, Takaoka, Japan) using the protocols supplied by the manufacturer.

1 µg (60 µl) of sheared input gDNA was used according to the protocol supplied by the MethylMiner kit (Invitrogen).

For total RNA isolation, the TRIzol Reagent (Life Technologies, USA) was used according to the protocol supplied by the manufacturer.

Next, treated cells were processed using the protocol as supplied by the manufacturer (Merck, Calbiochem, EMD Chemicals Inc., Darmstadt, Germany).