Sentence examples for using the protocol recommended from inspiring English sources
An LR recombination reaction between pENTR-HiDXS and a destination vector, pDEST17 (Invitrogen), was performed to generate an expression plasmid, pDEST17-HiDXS, using the protocol recommended by the supplier (Invitrogen).
For binding studies using fluoroproteoliposomes, fluorescein-4-isothiocyanate (Dojindo, Tokyo, Japan) was labeled with phosphatidyl serine using the protocol recommended by the manufacturer and was then embedded with membrane proteins prepared from U937 cells pretreated with 150 nM PMA for 4 days.
Pretreatment using the protocol recommended here would result in the remaining S0 impurity constituting only ~0.2% of the complete oxidation products.
The same device was used to monitor EVLW based on the number of B-lines or ultrasound lung comet tails (ULC) counted on images [27 29] using the protocol recommended by Gargani et al. [28].
Cells were harvested and total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA), using the protocol recommended by the supplier.
DNA was extracted after harvesting the cells by trypsinization followed by DNA extraction using QIAamp DNA mini kit (Qiagen Inc, Mississauga, ON, Canada), using the protocol recommended by the supplier.
RNA amplification, cDNA labelling and hybridization from XTC.UC1 cells were performed using the protocols recommended by the Transcriptome Core Facility (http://cardioserve.nantes.inserm.fr/ptf-puce/).
Lung function tests were performed using the protocols recommended by ATS/ERS guidelines [ 10].
The titer and percentage of recombinant phages in the library were determined using the protocols recommended by the manufacturer.
IL-5 and IL-13 concentrations were determined by two-site sandwich ELISAs, using the protocols recommended by the antibody suppliers.
Commercial ELISAs (USCN, Wuhan, China) targeting potential severity markers were used to measure in duplicate the levels of the proteins in individual DF and SD plasma specimen, using the protocols recommended by the manufacturer.
