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The second strategy consists in generating the fingerprint of each user according to a Tardos code, exploiting the fact that such codes can be securely distributed using the protocol proposed in [33].
Fresh G. americana leaves were collected from a single individual to be used in the initial DNA extraction, using the protocol proposed by Doyle and Doyle (1990).
Total genomic DNA was extracted from fresh leaves collected from a single C. legalis tree using the protocol proposed by Doyle and Doyle (1987).
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The 3'/5' ratios obtained were dramatically above the cut-off values when targets were synthesised using the protocols proposed by Arcturus, Ambion, and Epicentre but remained acceptable (below 10) for cDNA targets prepared according to the Nugen method.
That way, future studies may directly use the protocol proposed in this work.
For identification of Trichinella genotypes the random amplified polymorphic DNA analysis (RAPD) was used following the protocol proposed by [ 1].
Calibrations were performed using a protocol proposed by Zeintl et al. [8].
The protocols proposed in this paper use the hierarchical model concept to reduce the communication overhead and provide secure group communications well suited for combined wired/wireless networks.
Reflecting its wide use, the simplified protocol proposed by Smits et al [4], which involves avoiding the injection of PBS in the opposite patagium as an unneeded control [2], has been cited ca. 200 times since its publication in 1999 (ISI web of Science, acceded on August 2008).
The time bound to build network topology using the proposed protocol is also estimated by computer simulation.
We show execution schedules achieved using the proposed protocol and highlight its advantages and flaws when applied to our underwater scenario.
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