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LSF images were collected using the protocol of Kaye et al.1.1
Using the protocol of Lancaster et al.,40 in which the first step, an efficient generation of EBs, was modified as in Brennand et al.,33 we successfully generated cerebral organoids from hESCs (H9 and HUES8 lines) and from seven human iPSC lines.
ABTS radical cation assay was carried out using the protocol of Re et al.[45].[45]
Docking simulation of FXa was performed using the protocol of GOLD.
Total flavonoids content was determined by using the protocol of Sakanaka et al.[38].[38]
Cardiovascular fitness was assessed with an incremental test on a bicycle ergometer using the protocol of Kuipers et al. [12].
Using the protocol of Milanova et al. (2006), ground defatted meal (20 g) was mixed with 200 ml of 0.6 M NaCl solution.
The ligation of 1 and 2 was performed using the protocol of hydrazide based native chemical ligation (Fang et al., 2011; Pan et al., 2016).
Carboxypeptidase A activity was measured using the protocol of Folk and Schirmer (1963) using HPA (hippuryl-l-phenylalanine) as substrate (dissolved in 25 mM Tris HCl, 10 mM CaCl2 buffer, pH 7.8).
Total DNA was extracted using the protocol of Harrison et al. [27] with the following modifications.
A PCR was carried out using the protocol of [10] to confirm that collected females are An.
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