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The test graphs are generated using the protocol defined in [26].
Therefore, using the protocol defined here and the publicly available genotyping data of this analysis (Hippolyte et al., 2011), there is an opportunity to compare local collections with this broad Musa sample as reference.
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In addition, using the protocol-defined AE criteria set a priori (>10% increase in BMI between visits), excessive weight gain occurred in 9.0% of the M + R group compared with 4.3 and 7.3% in the M and M + L groups, respectively (not statistically significant).
PCR was conducted by using the PCR protocol defined above with high-fidelity Pfu DNA polymerase (Promega, Madison, WI, USA) and 4 pairs of specific primers (sequences available upon request).
The protocol defined independent dose reduction criteria.
MS was defined using the protocol specifically designed for Asian Indian population.
using the protocol provided by the supplier.
The hybridized slides were scanned with a ScanArray Express Microarray Scanner, Packard Bioscience/PerkinElmer (Shelton, CT) and the fluorescence was quantified by using the ScanArray Gx/ProScanArray, Microarray Analysis System (Packard biosciences/Perkin Elmer, Shelton, CT) using a protocol defined by a corresponding GAL file previously generated.
Passive biomechanics were tested using bi-axial tensiometry by a plunger test on a 500-N Zwick tensiometer with a 200-N cell load (Zwick GmbH & Co. KG, Ulm, Germany) using a protocol defined earlier [18].
Paired end 50 base sequencing by synthesis was performed using TruSeq SBS kit v3 (Illumina) on an Illumina HiSeq 1000 using protocols defined by the manufacturer.
A sensitivity analysis will be performed using the per-protocol population defined as those patients not deviating from the protocol in such a manner that the assessment of efficacy endpoints may be biased.
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