Sentence examples for using the protein synthesis from inspiring English sources

Secondly, when the consolidation processes in the OB were pharmacologically blocked using the protein synthesis inhibitor anisomycin [36], then the spaced learning failed to promote long-term retention of the task and enhanced neurogenesis.

The role of de novo protein synthesis was evaluated using the protein synthesis inhibitor cycloheximide (CHX).

We first measured the degradation rate by blocking new protein translation using the protein synthesis inhibitor cycloheximide.

To assess other possible causes for the differential behaviour of the distinct caspase-10 isoforms, their stability was measured using the protein synthesis inhibitor cycloheximide (CHX).

We have then determined Yap8 stability in the presence and absence of As by using the protein synthesis inhibitor cycloheximide (CHX).

To check whether the reduced stability of BACE1 by CHIP is a post-translational event, we first examined half-life of BACE1 protein using the protein synthesis inhibitor cycloheximide (CHX).

To determine whether infection impairs the formation of new PBs, or induces the disassembly of existing PBs, we used the protein synthesis inhibitor puromycin.

In order to show that the p53 degradation by HPV E6 in this assay was a post-translational event, we used the protein synthesis inhibitor cycloheximide (CHX) to estimate the half-life of p53 in the presence or absence of co-expressed E6. 293T cells were co-transfected with HA-p53 and E6 ORFs from HPV11, HPV18, HPV53, HPV56 or HPV61.

To thoroughly examine if the increases in protein and expression levels of E-cadherin and β-catenin were associated with upregulation in transcription, we used the protein synthesis inhibitor puromycin.

For the antibody mimics, we chose to study the tandem 10FN3 monobody HA4-7c122), which binds with nanomolar affinity to the Src homology 2 (SH2) domain of the oncoprotein Bcr-Abl.[ 2f] First, we sortagged this tandem monobody to LFN-DTA (LDv5) and used the protein synthesis inhibition assay to study the variant's translocation efficiency in chronic myeloid leukemia (CML) K562 cells.

Coding regions of GFP, Pfs25 and CSK were individually cloned into each one of these two vectors, and then the resultant recombinant plasmids were used for the protein synthesis using the cell-free system.