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KerA showed 65% similarity to alkaline protease precursor (GeneBank: ADP23919.1), using the protein database of B. polyfermenticus.
The HBc sequences of genotypes B and C were attained using the protein database from NCBI and a total of 171 HBc sequences of genotype B and 159 HBc sequences of genotype C included.
Repetitive element proteins were identified using the protein database of repeats.
Typically, detection of protein sequences in collision-induced dissociation (CID) tandem MS (MS2) dataset is performed by mapping identified peptide ions back to protein sequence by using the protein database search (PDS) engine.
pekinensis were predicted using the protein database TrEMBL (http://www.ebi.ac.uk/uniprot/TrEMBLstats/), Iprscan (http://www.ebi.ac.uk/Tools/pfa/iprscan/), UniProtKB (http://www.ebi.ac.uk/uniprot/), GO (http://www.geneontology.org/), and KEGG (http://www.genome.jp/kegg/); the BLASTP E-value was set as 1 × 10−5.
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Using the protein databases EchoLocation [ 37], Uniprot [ 38], and Ecocyc [ 39] as well as the bioinformatic programs PSORTb [ 40] and TMHMM [ 9], the 1,568 pORFs included in the reconstruction were assigned to compartments.
When we used the protein database to match to metabolic function, we found 1.0% of the sequences in each sample matched to nitrogen metabolism, with a total of 446 sequences found in the tidepool mussels and 445 in the bench mussels.
Subsequent protein sequence analyses using the Protein Families database of alignments and Interpro database [ 33] revealed that the deduced amino acid sequences have characteristics common to rhodopsin GPCRs.
Second, we identify putative protein targets for each drug using the protein structure database and in-silico virtual docking.
Protein ontology was determined using the protein reference database [ 32].
The performance of all the proposed methods is analyzed using the protein sequence database from the Protein Information Resource (PIR) center.
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