41 Proteinuria will be measured using the urine protein to creatine ratio or by measuring the 24 h urinary protein excretion.
In cell culture, extracellular creatine was inversely correlated with the intracellular phosphocreatine/creatine ratio, suggesting that elevated plasma creatine may signal a low energetic state in tissues using the creatine phosphate shuttle.
Using the same strategy, human creatine kinase (hCK) and mouse G6PI (mG6PI) was produced.
Frozen crushed ventricular tissue or cells were prepared for the measurement of CK activity and/or the quantification of creatine by HPLC, and then normalized to protein content using the Lowry method as previously described.
Complete blood count, fibrinogen, and the biochemical profile [alanine aminotransferase (ALT) and aspartate aminotransferase (AST), bilirubin, creatine kinase (CK), creatinine, glucose, and C-reactive protein (CRP)] were assessed using the local hospital laboratory.
Urinary creatine was measured using the modified Jaffé method (Colorimetric Assay, Roche Diagnostics).
The creatine kinase (CK) assay was carried out using the CK-UV kinetic kit from Sigma.
Albumin, urea, globulin, total protein, β hydroxybutyrate (βHB), and creatine kinase (CK) were measured on an automated analyser (Olympus AU 400, Japan) using the reagents supplied by Olympus.
Pectoral and thigh muscles mRNA expressions of Atrogin1, Creatine kinase (CK), avian Uncoupling protein (avUCP), Deiodinase, iodothyronine, type III (DIO3), Deiodinase, iodothyronine, type II (DIO2) were evaluated using the semi-quantitative real time RT-PCR analyses.
The principal nonhormonal supplements are protein, creatine and vitamins [ 2].
