Sentence examples for using the protein array from inspiring English sources

The analysis was carried out using the protein array technology [11], which allows high throughput analysis of human sera against a large number of antigens.

Using the protein array assay and specific Abs to deplete angiogenesis factors, we were able to identify whether angiogenesis factors released by BM have a role in human islet survival.

Because RelA is the only SETD6 substrate known to date, we used the protein array to identify additional substrates of SETD6.

As a proof of principle, we first used the protein array system to validate known substrates for the well-defined PKMT enzyme SETD7 [ 11- 13].

In this study, we used the protein array platform and developed two independent screening methods for the identification of new substrates of PKMTs.

As many commercially available pan-methyl antibodies crossreact with unmodified or non-methyl-modified sequences, we used the HEMP platform [ 15] to carefully characterize the antibody that was used for the protein array experiments.

Five SLE patients were used for a pilot study using the screening protein array, as described below in the section on protein array.

Human neutrophils were stimulated under stirring at 37○C for 1 min with the classical chemoattractant fMLP (100 nM) and protein phosphorylation was evaluated by using the Kinexus protein array service (www.kinexus.ca) (phosphorylation data are available in Supplementary Material: PMN-PhosphoSer.NA, PMN-PhosphoTyr.NA, PMN-PhosphoThr.NA).

The use of the protein array also bypassed the time-consuming sample collection step especially for tissue samples from human volunteers.

Finally, each set of experiments consisted of three independent microarray assays, including one GST replicate (negative control) and two replicates of each of the on-chip enzymatic assays using the corresponding PKMT on the protein array For SETD6, the set of positive hits was considered to be the union of the sets of positive hits for each of the two PKMT protein array replicates.

Second, for many proteins on the protein array used, the spotted protein does not cover the full-length sequence, so some positive hits may be missed.