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A median-joining haplotype network for the same dataset was also built using the program NETWORK.
Median joining networks were constructed using the program NETWORK, ver. 4.1.1.2 [66].
MJN of the S-gene quasispecies of HBV/E and HBV/A3 were constructed using the program NETWORK 4.0 [26].
Network analysis using the program Network 4.5.0.0 (http://www.fluxus-engineering.com) was performed on MLST sequences and on FAFLP data.
To visualize the relationships among haplotypes we inferred a median-joining network [71] using the program Network, v. 4.5.1.0 (available at www.fluxus-engineering.com) with varying genetic distance parameter epsilon (e = 0, 10, and 20), and equal weights for transitions and transversions.
The NADH5 and cytochrome b regions were combined for each species (jungle cat: 460 bp NADH5, 141 bp cytochrome b, leopard cat: 302 bpNADH5, 202 bp cytochrome b) and genetic structure was assessed through median-joining haplotype networks [31] using the program NETWORK (version 4.5.1.0; http://www.fluxus-engineering.com).
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Digital images from the tissue sections were captured and computerised planimetry was performed using the program Picsara (Euromed Networks, Stockholm, Sweden).
The MJ network was computed with the program Network 4.5.0 (http://www.fluxus-engineering.com) and the SP network was inferred using the program Tcs 1.21 [ 49].
A haplotype network constructed using the program TCS with the POMC data shows four separate networks and similar patterns to the phylogenetic tree results.
To evaluate relationships among sequences, we constructed a phylogenetic network using the program SplitsTree4 [ 68].
We calculated the secondary distance tree of the network using the program UVCLUSTER (Arnau et al. 2005) and then we used the program TreeTracker (Marco and Marín 2007) to compare the hierarchical representation of the interactome with the dichotomic partition (duplicate or nonduplicate) of the mitochondrial gene set.
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