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AMOVA (Analysis of Molecular Variance) was performed using the program implemented in ARLEQUIN.
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A three-dimensional model of the selected putative proteins was generated using the programs implemented by the automated protein homology-modeling server SWISS-MODEL (Swiss Institute of Bioinformatics [ 24]).
The DNA sequences were aligned using the program MUSCLE implemented in the software MEGA5 (Tamura et al. 2011) with minor manual adjustments.
and then sequenced using an ABI PRISM dye terminator cycle sequencer, model 3700 (Applied Biosystems, Foster City, California, U.S.A .. DNA sequences were aligned using the program MUSCLE implemented in the software MEGA5 (Tamura et al.2011) with subsequent manual adjustments.
The structure solution was obtained by molecular replacement with carbonmonoxy horse hemoglobin (PDB-code: 2D5X) as starting model, using the program PHASER implemented in the CCP4 suite [31].
Cumulative behaviour of the het-C alleles was determined using the program SNAP implemented as a web site interface (http://www.hiv.lanl.gov/content/hiv-db/SNAP/WEBSNAP/SNAP.html) based on Nei and Gojobori method [61].
The dendrogram was visualized using the program TREEVIEW implemented in NTSYSpc.
Note: a, Codon sites under positive selection that are estimated using the program CODEML implemented in the PAML package v4.4.
Codons of insect sequences of usp and cox1 genes were aligned using the program Muscle implemented in SeaView [ 64].
The effective number of codons used in a gene (Nc), a measure of the codon bias, was assessed using the program "chips" implemented in the EMBOSS package [ 57].
The effective number of codons used in a gene (Nc), a measure of the codon bias, was assessed using the program "chips" implemented in the EMBOSS package [ 72].
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