Approximately 2×106 cells were transfected (Effectene, Qiagen) with either myc- or HA-tagged FraWT, FraΔP1, FraΔP2, and FraΔP3 using the procedure recommended by the manufacturer.
Fatty acid methyl esters (FAME) were identified using the procedure recommended by Microbial Identification System (MIDI, Sherlock Microbial Identification System Version 4.0, MIS Operating Manual, March 2001, Newark, DE).
using the procedure recommended by the manufacturer.
The date of death was determined using the procedure recommended by the GPRD.
CORT levels were measured with a commercially available ELISA kit (Cayman Chemical, MI) using the procedure recommended by the manufacturer.
The Greek version of the CIS-R was translated and back-translated using the procedure recommended by the World Health Organization http://www.who.int/substance_abuse/research_tools/translation/en/index.html.html
Scores are transformed, using the procedure recommended by Kohout et al to make it compatible with the full 20-item instrument.
DNA was isolated from 1.5 × 107 CAL 27 and CAL 27-TF16 culturedultured with and without PAC, with the GenomicPrep DNA isolation kit (Amersham Biosciences: Buckinghamshire, UK), using the procedure recommended by the manufacturer.
To assess cumulative effects due to repeated exposure to poverty, we conducted latent class growth modelling to identify longitudinal patterns of poverty over the four survey rounds under study, using the procedure recommended by Muthén and Muthén [ 43] (p. 185 220).
To determine if any samples harbored the HPV virus, DNA was isolated from the saliva sample using a minimum of 3.5 x 10 cells using the GenomicPrep DNA isolation kit (Amersham Biosciences: Buckinghamshire, United Kingdom), using the procedure recommended by the manufacturer as previously described [ 12, 42, 78, 79].
