Differences between the coefficients were tested after Fisher's z-transformation [ 22], using the procedure provided by Goldstein [ 23].
Total RNA were extracted using the procedure provided with Quick Prep® total RNA extraction kit (Amersham Pharmacia Biotech, Piscataway, New Jersey) as described before [ 6, 9].
The Integrated Hospital System (Sistema Hospitalar Integrado; SHI) and the INCA Absolute System databases were used to record the procedures provided to women with breast cancer.
Also invariant recognition can, in principle, be tested using these procedures, provided that the stimuli shown to the animals are independently transformed in each trial.
The samples were then stained using an ABC staining system following the procedures provided by the manufacturer (Santa Cruz).
Plasmids containing cDNMT3B 3'UTR and miRNA were prepared in a 100-mL LB culture of transformed XL1-Blue E. coli using an EndoFree Plasmid Maxi kit (Qiagen, Valencia, CA, USA) and cotransfected into 293 FT cells using the transfection procedures provided by Invitrogen.
After mixing the discrete samples, scattering data were acquired for 5 min and reduced using the standard procedures provided by NIST.
Total microbial genomic DNA was extracted from 5-g compost samples using the standard procedure provided by the Ultra Clean Mega Soil DNA Kit MO BIO Laboratoriess, Carlsbad, CA).
Supernatant (25 µl) was used for ATP determination following the procedure provided in the Sigma FL-AA Bioluminescent assay kit.
Total RNA was extracted from wheat tissues using TRI reagent™ according to the procedure provided by the manufacturer (Molecular Research Center, Inc., Cincinnati, OH, USA).
