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Approximately 2×106 cells were transfected (Effectene, Qiagen) with either myc- or HA-tagged FraWT, FraΔP1, FraΔP2, and FraΔP3 using the procedure recommended by the manufacturer.
Fatty acid methyl esters (FAME) were identified using the procedure recommended by Microbial Identification System (MIDI, Sherlock Microbial Identification System Version 4.0, MIS Operating Manual, March 2001, Newark, DE).
using the procedure recommended by the manufacturer.
The date of death was determined using the procedure recommended by the GPRD.
CORT levels were measured with a commercially available ELISA kit (Cayman Chemical, MI) using the procedure recommended by the manufacturer.
The Greek version of the CIS-R was translated and back-translated using the procedure recommended by the World Health Organization http://www.who.int/substance_abuse/research_tools/translation/en/index.html.html
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Moderated mediation models were also explored using the procedures recommended by Preacher, Rucker, and Hayes (2007).
Ub-PESTc was radiolabeled with NaI using IODO-BEADS (Pierce) by following the procedure recommended by the manufacturer and Markwell (22).
Evidence for positively and negatively selected sites was sought using ADAPTSITE (version 1.3) according to the procedure recommended by Suzuki [ 21].
Cytokine secretion in the culture medium was assayed using an ELISA kit according to the procedure recommended by the supplier (CXCL10 (R&D System, #DIP100), IL-6 (R&D System, D6050), IL-10 (R&D System, D1000B), CCL18 (Abnova, #KA1757)).
Since the distributions of the volumes of PPH were not normal, these parameters were analyzed using the nonparametric procedure recommended by Conover and Iman [ 19].
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