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In both cases, specificity was assessed by using the probes described above.
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These observations prompted us to dynamically image microtubules, using mCherry-α-tubulin, and the Golgi apparatus, using the probe described above, simultaneously.
IAPs were detected using the probe described previously (Michaud et al., 1994).
The ES cells with the correct recombination were confirmed by standard Southern blot analysis using the probe described above.
When using any of the probes described earlier, amplifications or deletions of the specific chromosomal region are also detected.
All qPCR data in this manuscript were derived using the probes as described in Figure 1.
Based on the mean coverage obtained across the 24 samples included in this study (717×), it should be theoretically possible to multiplex as many as 344 samples on a single lane of the Illumina GAIIx to obtain ∼50× coverage following plastid enrichment using the probe set described here.
Another hybridization experiment was performed on muscle tissue from each mussel species as a negative control using the same probes described above.
In this in vitro homology search array, probes were designed, using the probe design method described in Ogura et al. [ 15], to estimate distantly related homologs of molluscan eye genes.
The DNA was then transferred to a nylon membrane for Southern blotting using the 5' external probe described above for ESCs screening.
SFPs were detected using the probe affinity shape power method described by Xu et al. [ 27].
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