As shown in Figure 5E, a specific band was detected using the primers (−582 567), suggesting that Ctnnb1 is associated with the Tcf4 sites.
Quantitative RT PCR using these primers suggested that all three chemokines were induced by DMXAA in the stromal cells but not significantly in the A375 melanoma cells (Supplementary Figure 3).
For in vitro transcription, each cDNA was cloned out from a mouse brain cDNA library using the primer sets suggested in the in situ hybridization data portal from the Allen Institute for Brain Science.
The β2S1 isoform on the other hand has hitherto escaped detection probably because it became detectible only using PCR primers suggested by the minigene results.
The coding sequence was cleanly obtained by using the primer combination LCOHym-Pat, suggesting that either only the COI gene was transferred to the nuclear genome, or the tRNA portion of the numt had undergone more extensive change.
Genomic PCR using Nipponbare primers suggested that many of the eight serpin genes tested are similar in the rices examined to those in Nipponbare.
Subsequent PCR amplification using more distal primers suggested the presence of extra sequence in the SHR compared to the WKY (see Figures 1b and 1c).
IgVH clonality analysis was done in DNA extracts from patients' blood samples using the suggested primers by the European Collaborative Study BIOMED 2 [ 23].
Primers were designed using the Primer Premier 5 software.
Primers were designed using the Primer 3 software (http://frodo.wi.mit.edu/primer3).edu/primer3
