Sentence examples for using the primers specified from inspiring English sources

The presence of resident prophages was screened by multiplex PCR using the primers specified in Additional file 5: Figure S1.

Sequencing and SNaPshot reaction for genotyping of SNP markers were also carried out on ABI-3130 according to the manufacture instructions using the primers specified in Table 1.

To express RPS10A, RPS10B, RPS10C cDNAs under the RPS10B promoter, RPS10B promoter region was amplified from Columbia wild-type genomic DNA, and the RPS10A (At4g25740.1), RPS10B (andg41520.1) and RPS10C (At5g52650.1) coding regions were amplified from Columbia wild-type cDNA using the primers specified in Table  5.

A 1 μg portion of total RNA was reverse transcribed (M-MLV Reverse Transcriptase, Promega, Germany) and PCR was carried out from several targets using the oligonucleotide primers specified in Supplementary Table IX.

pPrd1α, pHnt2α, pMge1α, pFmp40α, pGlo4α, pGpd2α, pLpd1α, pAep1α, pYgr031wα, Fmp36α, Mss116α, Acn9α, Mrpl11α and Gcv3α were created by amplifying the corresponding ORFs by PCR with the primers specified in Table S5 and using yeast genomic DNA as the template.

To construct RIVET reporters, putative promoters of interest were PCR amplified using primers specified in Table S2 using genomic DNA of S. enterica sv Typhimurium 14028 as a template.

For transmission analysis, seedlings were harvested after 12 days and genotyped using primers specified in Supplementary file 1.

For the generation of a Col PEG2::PEG2 construct, At1g49290 including 1.5 kb of upstream secuence was amplified by PCR using primers specified in Supplementary file 1.

Primers targeting the immune candidate genes were designed using the Primer-Blast [ 28] from NCBI http://www.ncbi.nlm.nih.gov/, specifying an expected PCR product of 200-300 bp and primer annealing temperatures between 56°C and 58°C.

Primers were designed using the Primer Premier 5 software.

Primers were designed using the Primer 3 software (http://frodo.wi.mit.edu/primer3).edu/primer3