Sentence examples for using the primers shown from inspiring English sources

PCR reactions were performed with Taq polymerase (Takara Biotechnology, Dalian, China) using the primers shown in Table S1.

In this study, the whole gene fragment excluding an original signal peptide of 19 amino acids (AAs), was amplified from the xylan-induced cDNA of strain Z5 using the primers shown in Additional file 1: Table S1, and was then inserted into pPICZαB to obtain the expression vector of pPICZαB-Xyn10A, which was next transformed and successfully expressed in P. pastoris X33.

cDNAs were then subjected to PCR using the primers shown in Table 3.

RT-PCR analysis of TNFα, IL-6, and TLR4, using β-actin as a control, was performed using the primers shown in Table S2.

Specifically, 498 and 469 bp fragments were independently amplified from the upstream and downstream regions of the targeted gene using the primers shown in Table 1.

The most conserved sequence stretches were chosen as 5' and 3' targeting sites, and were amplified by PCR from ITG2F6 genomic DNA using the primers shown in Table 1.

3'UTRs were amplified by PCR using the primers shows in Table S1.

(Applied Biosystems) was used to design the primers shown in Table 1.

Transcript levels were measured using the primers pairs shown underneath.

Seven PCR amplicons were prepared from purified DNA extracted from a glycerol stock of the bacterial isolate by using the PCR primers shown in Table 1.

The denitrifying functional genes were amplified using the primer pairs shown in Table 1.