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The full length graX gene was amplified from S. aureus Mu50 genome using the primers provided in Table 1.
The selected DNA constructs were amplified by PCR using the primers provided in the Illumina library kit.
Semi-quantitative PCR detection of TRM4A, TRM4B, NSUN5, NOP2B and NOP2C mRNA was performed using the primers provided (Additional file 1: Table S6).
The ends of the inserted PCR product were sequenced using the primers provided with the cloning kit for additional verification of FIV Ple cloned products.
Any wheat construct containing one of the two standard sequence elements can be screened using the primers provided, and the example of rice indicates that the assay can easily be adapted to other plants.
The first-strand cDNA used for 5' and 3'RACE were produced by using 1 μg of the infected RNA mix prepared for the SSH method, and using the primers provided in the kit.
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All reactions used the primers provided in Table 10.
We use the primers provided in the Agilent kits, but we order the Illumina adaptors oligonucleotides and primers directly from an oligonucleotide provider as we use excess amounts of these reagents.
Forester AP2-like protein mRNA sequence [GenBank: AY069953] as template and were used in combination with the primers provided by the kit.
The converted DNA was later enriched by 15 cycles of PCR using the Pfu Turbo Cx Polymerase (Agilent, Santa Clara, CA, United States), using the specific primers provided by Bios Scientific for enrichment.
The fidelity of the cloned product was verified by sequencing using the specific primers provided in the TOPO kit.
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