Sentence examples for using the primers presented from inspiring English sources

The distribution of alleles on the B. quintana chromosome is presented in Figure 3. Internal fragments of 531 622 length were amplified by using the primers presented in Table 4. PCR was performed in 50 µl volume as described previously [20].

PCR reactions to amplify targeted loci were performed using the primers presented in Supplementary Table S4.

Partial sequences of the genes atp6, cytb, nad4 and rrnS were determined using the primers presented in additional file 6.

WecA was amplified from E. coli K12 DNA using the primer sequences presented in the Supplementary materials.

The cDNA was generated using the ImPromII RT Kit (Promega, Madison, WI, USA) and the PCR amplifications were performed using the primer pairs presented in Table 1.

We found the four Y chromosome microsatellite loci from [29] to be easier to amplify and less sensitive to PCR conditions when using the modified primers presented here.

The initial analysis of the ASIC1 gene showed that at least the 5' end was present, when using the primers ASICMCF1, located in Exon 2 and beginning at base 89 of the coding sequence, and ASICMCR1, located in Exon 3 and ending at base 394 of the coding sequence.

As shown in Figure 1, the genomic DNA amplified by using the primer OPB-18 (5′-CCACAGCAGT-3′) presented a polymorphic band being approximately 700 bp in the susceptible strains only.

Using the primer sequences that are present in the data, short reads were first assigned to their respective amplicon.

RT template (cDNA) was subjected to PCR or real-time PCR amplification using primers presented in supplementary table S1, Supplementary Material online.

The products obtained are then amplified by PCR using two nested primers present in the linkers.