Sentence examples for using the primers outlined from inspiring English sources

Both FbiC and FbiAB ORFs were amplified using PfuUltra Fusion HS DNA polymerase (Stratagene) using the primers outlined in Table 2. FbiC was first cloned using NcoI/HindIII restriction sites and the FbiAB operon was subsequently cloned using NdeI/EcoRV restriction sites.

Cells were subsequently transfected with 1 nM of either EFNB2 esiRNA or scrambled esiRNA for 72 h, after which EFNB2 silencing was confirmed using RT-PCR using the same primers outlined in Table 1.

PCR amplicons for all target sequences were produced by monoplex conventional PCR using the primer sequences outlined below.

Details of all primers used in this study are presented in Additional file 1. PCR amplifications were carried out in a total volume of 20 μl as outlined in Ryynänen and Primmer [ 62] and using the primers and annealing temperatures outlined in Additional file 1.

Quantitative PCR was performed by sampling 5 μL of extracted sample using the primers, probe, and equipment outlined by Green et al. Interference created by high hemoglobin concentrations prevented polyacrylamide gel electrophoresis (PAGE) analysis of neat erythrocyte lysates; therefore, erythrocyte ghosts were analyzed for CAR expression.

Consensus primer sets were created for 24 of the 42 unique loci identified (57%) using the primer design criteria outlined above (Tables  1 & 2; full sequences of the loci are provided in Additional file 2).

Partial ITS1 of ribosomal RNA (ITSI-rRNA) gene of the parasites was amplified using the primers LITSR (5′-CTGGATCATTTTCCGATG-3′) and L5.8S (5′-TGATACCACTTATCGCACTT-3′) and PCR conditions outlined by others [ 19].

The cloning strategy is outlined in Figure 2. Briefly, a 1.53 Kb patatin B33 promoter fragment [ 20] was amplified from potato (cv Berolina) genomic DNA using the primers B33-up and B33-dw (Table 2).

These loci were amplified from the DNA extractions by polymerase chain reaction (PCR) using flourescently labeled primers outlined in the procedures of Heggenes et al. (2006).

WT CXCR4 and ΔT were obtained by PCR using primers outlined in Text S1, and cloned into the VTT plasmid to generate WT or ΔT fused to the TEV cleavage site ENLYFQL followed by tTA (plasmids WTCXCR4-tTA or ΔTCXCR4-tTA).

Primers were designed using the Primer Premier 5 software.