The expression levels of the key genes related to the biosynthesis of Pin, PMG, and PDG (4CL, GT, CHS) were examined using the primers listed in Table 1.
Genomic DNA (5 ng) was subjected to PCR using the primers listed in Table S1.
Converted DNA was amplified by PCR using the primers listed below.
For toeprinting experiments, in vitro transcribed lmo0302 RNA and chiA RNA was prepared using the primers listed in Table S3.
PCR was performed using the primers listed in Table 2, at a final concentration of 200 nM.
These also were not detected (Figure 1, Lanes D G), using the primers listed in Table 1.
qPCR was performed on a 1/1000 dilution of the cDNA using the primers listed in Tables S5 and S6.
The chloramphenicol resistance cassette (cat) of the pKD3 plasmid was amplified using the primers listed in Table 1.
DNA and cDNA were subjected to PCR and RT-PCR, respectively, using the primers listed in supplementary Table S6.
The 72-6/220 Pfcrt gene sequence was established as described [33], using the primers listed in Table S1.
Degenerate PCRs for amplifying gene fragments encoding seven subunits (β,γ, δ, ε, ζ, η, and θ) of CCT were conducted using the primers listed in Table S2.
