Sentence examples for using the primers in from inspiring English sources

Mutants were constructed using the primers in Table S1.

The mRNA expression was detected by RT-PCR using the primers in Table S1.

The presence of the insert in the vector and its orientation was assessed by conventional PCR and sequencing, as described above, using the primers in Table 3.

The mtDNA samples were amplified using the primers in 25 µl PCR reactions containing 50 µM dNTPs, 0.2 µM of each primer, 1 µl template, 2.5 µl Taq polymerase buffer, and 1 U Taq polymerase (TIANGEN, China).

qPCR reactions were performed with the ABI Prism 7900HT Fast Real-Time PCR System and Sequence Detection Systems Enterprise Database with 384 wells (Applied Biosystems Inc) with an absolute quantification template provided by the manufacturer, using the primers in Table 1, with GAPDH, Actin, and 18S as housekeeping genes to normalize the sample amount.

A SYBR Green assay was performed with SensiMix (Quantace) using the primers in Table S4.

The PCR amplification was performed using the primers listed in Supplementary Data 1.

Then, the coding sequence of Exo1 was replaced with other effector cDNAs using the primers listed in Supplementary Data 1.

Briefly, NGS libraries were prepared via two-step PCR using the primers listed in Supplementary Data 1.

Quantitative real-time PCRs were carried out using the primers listed in Supplementary Data 2 and iQ SYBR Green Master Mix (Bio-Rad).

The cDNA was PCR-amplified by rTaq (TAKARA) using the primers listed in Supplementary information, Table S1.