Using the primers and the missense polymorphism (rs1049434) described by Cupeiro et al. [9], we searched the sequence on gene bank.
One hundred eighty rice cultivars were genotyped at the qSW5 locus by using the primers, and they were divided into 3 types: Kasalath-type, Indica II-type, and Nipponbare-type (Song et al., 2011).
The same DNA preparations as used for rep-PCR were also used to amplify dnaK genes using the primers and protocol described previously (Stępkowski et al. 2003; Martens et al. 2007).
Both assays were performed using the primers and probe described previously [15], [18].
PCRs were performed using the primers and the prepared human, mouse, and rat cDNAs.
Target regions were amplified by PCR using the primers and cycling conditions described in Table S2.
Other constructs were performed in two PCR steps using the primers and templates listed in Table S1.
All reporter genes, except for the lux operon, were PCR amplified using the primers and templates listed in Table 5.
The expression level of the CYP2C93 mRNA was measured as previously described [6] using the primers and a probe specific for CYP2C93 mRNA with the following modifications.
We also assessed in parallel the expression of presumptive miR-34-regulated target genes and proteins, using the primers and methods as we described recently [6].
The DRD4 48 bp VNTR was assayed by polymerase chain reaction (PCR) using the primers and reaction conditions, as described in [16].
