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These data were analyzed using the PRIMER software package (Plymouth Routines in Multivariate Ecological Research, version 6) [38].
Also using the PRIMER software package, we calculated a Bray-Curtis similarity coefficient based on presence/absence data to examine the similarity of species lists: (1) among sub-regions, (2) between the entire deep-sea region and the continental shelf, and (3) between deep-sea sub-regions and the continental shelf.
A dendrogram was also generated using the PRIMER software.
The sequences of nine miRNAs were obtained from high-throughput sequencing, and primers were designed using the Primer software.
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Primers were designed using the Primer 3 software (http://frodo.wi.mit.edu/primer3).edu/primer3
All specific primers were designed by using the primer analysis software (Oligo 6.6, Molecular Biology Insights).
All oligonocleotides were designed using the primer 3 software (http://frodo.wi.mit.edu/primer3/).edu/primer3/
Primers were designed using the Primer 3 software and the specific sequences are given in Table S1.
Primers for real-time PCR were designed on Mentha piperita available sequences using the Primer 3 software [63].
Primers for RT-PCR were designed using the primer design software Primer3 (Table S5), and then the sequence homology for related proteins was checked.
All primers were designed using the Primer 3 software.
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