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Additionally, a multiplex PCR reaction detecting HHV1-6 (including HHV-3, also called Varicella-zoster virus 1; HHV-4, commonly known as Epstein-Barr virus; and HHV-5, human cytomegalovirus) was performed by using the primer setup described by Tenorio et al. (3 ).
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Primers were designed using the Primer Premier 5 software.
Two PCRs were setup using the primers Cep123-S01 and Cep123 GS-Rv; and Cep123-AS01 with Cep123 GS-Fw (the sequences of these primers are in supplementary material Table S1).
All MD simulations were performed using the following setup.
Steadiness was measured after shoulder elevation using the same setup.
Holby City is shot using the single-camera setup.
Use the Setup Assistant to setup your wireless network.
After Reverse Transcription, cDNAs were amplified by PCR using the probe specific primers, coupled to the T7 sequence for forward primers and the SP6 sequence for reverse primers.
Use the best control setup.
Use the nail primer.
Use the setup in the picture for the previous step.
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