Sentence examples for using the primer set from inspiring English sources

The episomal vectors were detected using the primer set for EP4.

PCR product using the primer set, 2F and 2R, is a 1111-bp fragment between 3'cbs and zeocin (PCR2).

The V1 V3 hyper variable region of bacterial 16S rRNA genes was amplified using the primer set 27F and 519R (Xiong et al. 2014a).

For the bacterial 16S rRNA gene library construction, the amplification was performed using the primer set 10f and 1100r [Lane 1991].

The amplification of the fungal ITS1 region was performed using the primer set ITS1-F [23] and Neo-qPCR Rev [24].

We constructed an FMP21-overexpressing strain based on a previously reported method (Hasegawa et al. [2012]) using the primer set listed in Table 1.

Eukaryotic 18S rRNA gene was amplified using the primer set Euk1a and Euk516r-GC [22] with the DF Taq DNA polymerase (Genaxxon bioscience GmbH).

HAA1-overexpressing strains were constructed on the basis of a previously reported method (Hasegawa et al. 2012) using the primer set listed in Additional file 1: Table S1 in the supplemental material.

A DNA fragment containing the S. cerevisiae XK gene (XKS1) was amplified from plasmid pRS406XKS (Madhavan et al. 2009a) as a template using the primer set TDH3-XK F (5′-CCGCACCAGTTCTCACACGGAACACCACTA-3′) and TDH3-XK R (5′-CCACCGCGGTCAATCAATGAATCGAAAATG-3′).

A DNA fragment containing the Orpinomyces sp. XI gene (xylA) (Madhavan et al. 2009a, b) was amplified using the primer set XI F (5′-ATTGAATTCATGACTAAGGAATATTTCCC-3′) and XI R (5′-AATGTCGACTTATTGGTACATGGCAACAA-3′).

The PCR amplification of the partial 16S rRNA genes was carried out using the primer set 27F-5′-AGA GTTTGATYMTGG CTC AG-3′, and 515R 5′-TACCGCGGCKGCTGGCA C-3′.