Sentence examples for using the primer pair from inspiring English sources

We amplified the circularised DNA using the primer pair of sgRNA-Rv and sgRNA-Fw, AAATAAGGCTAGTCCGTTATCAACTTGAAAAAG.

The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome (TAC) library derived from Z. latifolia.

The pyruvate kinase gene pyk was amplified by PCR from the chromosomal DNA of L. bulgaricus CAUH1 using the primer pair: forward 5′- CATGCCATGGGAACGAAGATTGTTAGTACTTTAG-3′ and reverse 5′- CCGAGCTCGCAATCCTAGATTACAGGTTTG-3′.

Subsequently, the 5′-RACE fragment was amplified by PCR from the cDNA product using the primer pair: Nested Universal Primer A (5′-AAGCAGTGGTATCAACGCAGAGT-3′) and a gene-specific primer Internal-R (5′-GCTCAATACCAGCCAGCTGTCCTTC-3′).

The DNA was amplified using the primer pair, LCO1490 and HCO2198 (Folmer et al. 1994).

The clones were screened by lysate PCR using the primer pair PorfXF and yqhDR (Table 2).

16S rRNA genes of Geobacteraceae bacteria were amplified using the primer pair Geo494F and Geo825R (Holmes et al. 2002), while those of total bacteria were amplified using the primer pair 341f and 534r (Watanabe et al. 2001).

For insertion of aphA, the gene was amplified from vector pMB03 using the primer pair KanR_A/KanR_B.

The 3' flanking region of the Aaku80 gene, which formed DRs, was amplified from A. aculeatus genomic DNA using the primer pair aku80CF1 and aku80CR1.

Using the primer pair TgsX-up and TgsX-down, products of ~800 bp are expected to be amplified from typical WS/DGAT coding sequences.

Transformants generated using the CRISPR Cas9 system were screened by colony PCR using the primer pair P_Km01-010 + P_Km01-010-011 (Additional file 1: Table S1).