Sentence examples for using the primer combinations from inspiring English sources
PCR was performed using the primer combinations: sense, 5'-TGTGTGCGGAAAGTATGCTGTC-3' and antisense 3'-RACE AAGCAGTGGTATCAACGCAGAGT.
Diagnostic PCRs for the integration event were performed for the 3' and 5' integration site using the primer combinations b3D-1/Pb-11 b3D-1/Pb-11 b3D-1/Pb-11 1183015 bp), respectively (for primer sequences see Tande Pb-10/b3D-3 Pb-10/b3D-3 Pb-10/b3D-3 1015
We generated the N-terminus deletion constructs lacking the first 128 pEGFP-129KLF66), 56 (pEGFP-57KLF6) and 16 (pEGFP-17KLF6) amino acids (aa), using the primer combinations fwd-129-283/rev-KLF6pCIneo, fwd-57-283/rev-KLF6pCIneo and fwd-17KLF6/rev-KLF6pCIneo fwd-17KLF6/rev-KLF6pCIneo fwd-17KLF6/rev-KLF6pCIneo
The figure depicts reliably amplified products using the primer combinations instead of amplified products from individual miRNA primers or SRAP primers.
A nested PCR was carried out using the primer combinations Full-cDNA5'-F/Full-cDNA3'-R and 277-F/277-R.
The L. donovani and L. major META1 genes were amplified from genomic DNA by using the primer combinations TV494 & TV757 and TV494 & TV758 respectively and GFP was amplified using TV276 & TV277 from pX63- EGFP vector.
The latter was amplified by PCR using the primer combination SIII5'-ATGGGAGGTATTCCGCCAC-3and3') and SIII3' (5'-AGGGCTTTAACCAGCTGCTC-3') before labeling.
Subsequently, the deletion fragment was generated by PCR using the primer combination F_upclpK/R_dwnclpK and equimolar amounts of all three fragments as template.
For the mtDNA CR approximately 450 base pairs (bp) of the first (left) domain of the mitochondrial control region was obtained using the primer combination of L15998 (5'-TAC CCC AAA CTC CCA AAG CTA-3'), with e H-strand primer CSBDH (5'-TgA ATT AGG AAC CAG ATG CCA G-3') [39].
Cytochrome oxidase 1 sequences were obtained using the primer combination of universal primers FishF1-5' TCACCCC AAC CAC AAA GAC ATT GGC and3' and FishR1-5' TACTACTCTCT GGG TGG CCA AAG AAT CA.-3' described in Ward et al., (2005) with the same PCR profiles and sequencing procedures as described above.
Nuclear ITS1 sequences were obtained following the protocol described in Chow et al. [11] using the primer combination of ITCC-F-5' TCC GTA GGT GAA ACC TGC GG-3' with the ITS1-R-5'-CGC TGC GTT CTT CAT CG-3' using the same reactive described above.
