Sentence examples for using the primer combination from inspiring English sources

Subsequently, the deletion fragment was generated by PCR using the primer combination F_upclpK/R_dwnclpK and equimolar amounts of all three fragments as template.

The latter was amplified by PCR using the primer combination SIII5'-ATGGGAGGTATTCCGCCAC-3and3') and SIII3' (5'-AGGGCTTTAACCAGCTGCTC-3') before labeling.

For the mtDNA CR approximately 450 base pairs (bp) of the first (left) domain of the mitochondrial control region was obtained using the primer combination of L15998 (5'-TAC CCC AAA CTC CCA AAG CTA-3'), with e H-strand primer CSBDH (5'-TgA ATT AGG AAC CAG ATG CCA G-3') [39].

Cytochrome oxidase 1 sequences were obtained using the primer combination of universal primers FishF1-5' TCACCCC AAC CAC AAA GAC ATT GGC and3' and FishR1-5' TACTACTCTCT GGG TGG CCA AAG AAT CA.-3' described in Ward et al., (2005) with the same PCR profiles and sequencing procedures as described above.

Nuclear ITS1 sequences were obtained following the protocol described in Chow et al. [11] using the primer combination of ITCC-F-5' TCC GTA GGT GAA ACC TGC GG-3' with the ITS1-R-5'-CGC TGC GTT CTT CAT CG-3' using the same reactive described above.

The first round of PCR was conducted using the primer combination of HBV1798FLong and HBV1801RLong (Table 1) as follows: initial denaturation (94°C for 3 min), followed by 10 cycles with each cycle consisting of denaturation at 94°C for 20 sec, annealing at 55°C −45°C for 30 sec, and extension at 68°C for 4 min. Annealing temperature was reduced at the rate of 1°C per cycle from 55°C to 45°C.

PCR was performed using the primer combinations: sense, 5'-TGTGTGCGGAAAGTATGCTGTC-3' and antisense 3'-RACE AAGCAGTGGTATCAACGCAGAGT.

Diagnostic PCRs for the integration event were performed for the 3' and 5' integration site using the primer combinations b3D-1/Pb-11 b3D-1/Pb-11 b3D-1/Pb-11 1183015 bp), respectively (for primer sequences see Tande Pb-10/b3D-3 Pb-10/b3D-3 Pb-10/b3D-3 1015

We generated the N-terminus deletion constructs lacking the first 128 pEGFP-129KLF66), 56 (pEGFP-57KLF6) and 16 (pEGFP-17KLF6) amino acids (aa), using the primer combinations fwd-129-283/rev-KLF6pCIneo, fwd-57-283/rev-KLF6pCIneo and fwd-17KLF6/rev-KLF6pCIneo fwd-17KLF6/rev-KLF6pCIneo fwd-17KLF6/rev-KLF6pCIneo

A nested PCR was carried out using the primer combinations Full-cDNA5'-F/Full-cDNA3'-R and 277-F/277-R.

The figure depicts reliably amplified products using the primer combinations instead of amplified products from individual miRNA primers or SRAP primers.